Journal: Journal of Nanobiotechnology

Article Title: Hemin-incorporating DNA nanozyme enabling catalytic oxygenation and GSH depletion for enhanced photodynamic therapy and synergistic tumor ferroptosis

doi: 10.1186/s12951-022-01617-0

Figure Lengend Snippet: Schematical showing the preparation of DF with AS1411 G4 motif for Ce6 loading and hemin incorporation for tumor targeting PDT/ferroptosis combinatorial therapy

Article Snippet: Hemin and Chlorin e6 (Ce6) were purchased from Frontier Scientific Co., Ltd (Utah, USA).

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: Hemin-incorporating DNA nanozyme enabling catalytic oxygenation and GSH depletion for enhanced photodynamic therapy and synergistic tumor ferroptosis

doi: 10.1186/s12951-022-01617-0

Figure Lengend Snippet: A Schematic showing the design of template sequence and the preparation of DF. B The TEM micro-images of DF and CH/DF. C The SEM micro-image of CH/DF. D DLS and E surface charge measurements of DF and CH/DF. Inset: the appearance of the nanoparticles after centrifugation. F The fluorescence spectra of Ce6 before and after loading into DF. G The elemental mapping micro-image of CH/DF

Article Snippet: Hemin and Chlorin e6 (Ce6) were purchased from Frontier Scientific Co., Ltd (Utah, USA).

Techniques: Sequencing, Centrifugation, Fluorescence

Journal: Journal of Nanobiotechnology

Article Title: Hemin-incorporating DNA nanozyme enabling catalytic oxygenation and GSH depletion for enhanced photodynamic therapy and synergistic tumor ferroptosis

doi: 10.1186/s12951-022-01617-0

Figure Lengend Snippet: A Ex vivo imaging the biodistribution of CH/DF and free Ce6 at 12 h post-injection into tumor bearing mice. B The intensity quantification at different organs based on the fluorescence images in A . C Dynamic monitoring the tumor growth after various treatments. D The appearance and E weight of the tumors at day 16 post various treatments. F Tumor H&E staining images, fluorescent staining of TUNEL and caspase-3 of the mice with different treatments

Article Snippet: Hemin and Chlorin e6 (Ce6) were purchased from Frontier Scientific Co., Ltd (Utah, USA).

Techniques: Ex Vivo, Imaging, Injection, Fluorescence, Staining, TUNEL Assay

Journal: IET Nanobiotechnology

Article Title: Mn‐doped ZnS quantum dots‐chlorin e6 shows potential as a treatment for chondrosarcoma: an in vitro study

doi: 10.1049/iet-nbt.2018.5387

Figure Lengend Snippet: TGA analyses of the QDs, QD‐ED and QD‐ED‐Ce6

Article Snippet: 1‐ethyl‐3‐[3‐dimethylaminopropyl] carbodieimide hydrochloride (EDC) were purchased from Sigma–Aldrich (Munich, Germany) and Chlorin e6 (Ce6) were purchased from Medkoo (USA).

Techniques:

Journal: IET Nanobiotechnology

Article Title: Mn‐doped ZnS quantum dots‐chlorin e6 shows potential as a treatment for chondrosarcoma: an in vitro study

doi: 10.1049/iet-nbt.2018.5387

Figure Lengend Snippet: Cell viabilities for free Ce6, free QD and QD‐Ce6 complexes (0.5 µmol l−1) before and after X‐ray treatment on SW1353 cells, respectively

Article Snippet: 1‐ethyl‐3‐[3‐dimethylaminopropyl] carbodieimide hydrochloride (EDC) were purchased from Sigma–Aldrich (Munich, Germany) and Chlorin e6 (Ce6) were purchased from Medkoo (USA).

Techniques:

Journal: Biomolecules

Article Title: Analysis of Self-Assembled Low- and High-Molecular-Weight Poly-L-Lysine–Ce6 Conjugate-Based Nanoparticles

doi: 10.3390/biom14040431

Figure Lengend Snippet: Preparation of LPL and PLCe6. ( a ) Schematic diagram of the structure and chemical synthesis of LPL; ( b ) measurement of the molecular weight of LPL with MALDI-TOF; ( c ) schematic diagram of the structure and chemical synthesis of PLCe6; ( d ) confirmation of the synthesis of LPL1Ce6 and HPL10Ce6 molecules from Ce6 with UV–vis spectroscopy after purification; ( e ) RP-HPLC of Ce6, LPL1Ce6 and HPL10Ce6.

Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (Waltham, MA, USA) and chlorin e6 (Ce6) was obtained from Leap Chem (Hong Kong).

Techniques: Molecular Weight, UV-Vis Spectroscopy, Purification

Journal: Biomolecules

Article Title: Analysis of Self-Assembled Low- and High-Molecular-Weight Poly-L-Lysine–Ce6 Conjugate-Based Nanoparticles

doi: 10.3390/biom14040431

Figure Lengend Snippet: Cellular uptake and cytotoxicity effects of PLCe6 nanoparticles. ( a ) Cellular uptake of PLCe6 nanoparticles in CT26.WT cells treated for 3 h was observed by using fluorescence microscopy (red; Ce6, λEx = 540 nm, λEm = 605 nm, blue; DAPI, λEx = 375 nm, λEm = 460 nm); scale bars = 50 µm for all. ( b ) Cytotoxicity evaluation of Ce6, LPLCe6, and HPLCe6 on CT26.WT cells for 24 h with laser irradiation; ( c ) without laser irradiation ( n = 6), mean ± SD, *** p < 0.001.

Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (Waltham, MA, USA) and chlorin e6 (Ce6) was obtained from Leap Chem (Hong Kong).

Techniques: Fluorescence, Microscopy, Irradiation

Journal: mSphere

Article Title: Antimicrobial Photodynamic Therapy with Chlorin e6 Is Bactericidal against Biofilms of the Primary Human Otopathogens

doi: 10.1128/mSphere.00492-20

Figure Lengend Snippet: Bactericidal effects of Ce6-aPDT on planktonic cultures (A) and established biofilms (B) of M. catarrhalis 7169 (Mcat), S. pneumoniae EF3030 (Spn), and NTHi 86-026NP (NTJi). Total light dosage was 12 J/cm 2 (100 mW/cm 2 , 2 min) using 1 μM Ce6 for all planktonic assessments and 90 J/cm 2 (100 mW/cm 2 , 15 min) using 10 μM Ce6 for biofilm assays. Control sets were exposed to Ce6 but not light (+PS–L), light in the absence of Ce6 (–PS+L), or were not treated (–PS–L). Survival of bacteria, both planktonic and biofilm-associated, was determined by viable plate counts. All bacteria exposed to aPDT treatment (+PS+L) were significantly decreased in viability compared to that of the controls (–PS–L) with a >99.9% kill rate. The dashed lines indicate the limit of detection. The data are presented as the means ± SDs, with individual data points shown. ns, not significant; * , P = 0.0327; *** * , P < 0.0001.

Article Snippet: High-purity (>98%) lyophilized chlorin e6 trisodium salt (Ce6) (Fotolon; Apocare Pharma GmbH, Bielefeld, Germany) was handled in the dark to restrict ambient light exposure.

Techniques: Control, Bacteria

Journal: mSphere

Article Title: Antimicrobial Photodynamic Therapy with Chlorin e6 Is Bactericidal against Biofilms of the Primary Human Otopathogens

doi: 10.1128/mSphere.00492-20

Figure Lengend Snippet: Viability of biofilm-detached bacteria (hatched bars) was assessed by evaluating surviving CFU in the surrounding saline supernatant following aPDT of M. catarrhalis 7169 (Mcat), S. pneumoniae EF3030 (Spn), and NTHi 86-026NP (NTHi) biofilms (solid bars). There were no surviving bacteria (≤10 CFU) detected in the surrounding saline following Ce6-aPDT of the biofilms compared to the untreated control groups (≥10 6 CFU), indicating that bacteria that detach from the biofilms in response to aPDT are not viable. The dashed line indicates the limit of detection. The data are presented as the means ± SDs, with independent replicates shown. *** * , P < 0.0001.

Article Snippet: High-purity (>98%) lyophilized chlorin e6 trisodium salt (Ce6) (Fotolon; Apocare Pharma GmbH, Bielefeld, Germany) was handled in the dark to restrict ambient light exposure.

Techniques: Bacteria, Saline, Control

Journal: mSphere

Article Title: Antimicrobial Photodynamic Therapy with Chlorin e6 Is Bactericidal against Biofilms of the Primary Human Otopathogens

doi: 10.1128/mSphere.00492-20

Figure Lengend Snippet: Representative SEM images (×5,000 magnification) of M. catarrhalis 7169 (A), S. pneumoniae EF3030 (B), and NTHi 86-026NP (C) biofilms before (left) and after (right) Ce6-aPDT treatment.

Article Snippet: High-purity (>98%) lyophilized chlorin e6 trisodium salt (Ce6) (Fotolon; Apocare Pharma GmbH, Bielefeld, Germany) was handled in the dark to restrict ambient light exposure.

Techniques:

Journal: mSphere

Article Title: Antimicrobial Photodynamic Therapy with Chlorin e6 Is Bactericidal against Biofilms of the Primary Human Otopathogens

doi: 10.1128/mSphere.00492-20

Figure Lengend Snippet: Efficacy of two sequential treatments of Ce6-aPDT was evaluated on biofilms of M. catarrhalis 7169 (A) and S. pneumoniae EF3030 (B). NTHi 86-026NP biofilms were omitted from this analysis because the single aPDT treatment was sterilizing. Biofilms were subjected to one round of Ce6-aPDT (1× aPDT at a total light dosage of 90 J/cm 2 using 10 μM Ce6; solid bars) or immediately retreated with a second round (2× aPDT) using the exact same treatment parameters (hatched bars). Dual treatment completely eradicated all viable biofilm-associated bacteria (≤10 CFU) compared to untreated controls (≥10 5 CFU) in both organisms. The dashed lines indicate the limit of detection. The data are presented as the means ± SDs, with independent replicates shown. ns, not significant; * , P = 0.0281; *** * , P < 0.0001.

Article Snippet: High-purity (>98%) lyophilized chlorin e6 trisodium salt (Ce6) (Fotolon; Apocare Pharma GmbH, Bielefeld, Germany) was handled in the dark to restrict ambient light exposure.

Techniques: Bacteria

Journal: mSphere

Article Title: Antimicrobial Photodynamic Therapy with Chlorin e6 Is Bactericidal against Biofilms of the Primary Human Otopathogens

doi: 10.1128/mSphere.00492-20

Figure Lengend Snippet: Ce6-aPDT is effective at preventing bacterial regrowth and biofilm reestablishment 24 h following dual treatment. Biofilms of M. catarrhalis 7169 (A), S. pneumoniae EF3030 (B), and NTHi 86-026NP (C) were exposed to a single (gray bars, open symbols) or dual (white bars, closed symbols) Ce6-aPDT treatments (total light dosage of 90 J/cm 2 using 10 μM Ce6). The surviving biofilm-associated bacteria were enumerated to assess viability immediately following treatment ( t = 0, solid bars) or 24 h posttreatment (t = 24 h, lined bars). The dashed lines indicate the limit of detection. The data are presented as the means ± SDs, with independent replicates shown. ns, not significant; ** * , P = 0.0008; *** * , P < 0.0001.

Article Snippet: High-purity (>98%) lyophilized chlorin e6 trisodium salt (Ce6) (Fotolon; Apocare Pharma GmbH, Bielefeld, Germany) was handled in the dark to restrict ambient light exposure.

Techniques: Bacteria